THE PROTECTIVE EFFECT OF SERUM AGAINST HYDRODYNAMIC DAMAGE OF HYBRIDOMA CELLS IN AGITATED AND SURFACE-AERATED BIOREACTORS

被引:51
作者
KUNAS, KT
PAPOUTSAKIS, ET
机构
[1] NORTHWESTERN UNIV, DEPT CHEM ENGN, 2145 SHERIDAN RD, EVANSTON, IL 60208 USA
[2] RICE UNIV, DEPT CHEM ENGN, HOUSTON, TX 77251 USA
基金
美国国家科学基金会;
关键词
Aeration; Agitation; Bioreactor; Hybridoma cell; Serum;
D O I
10.1016/0168-1656(90)90051-C
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The effect of serum on the growth rate and metabolism of CRL-8018 hybridoma cells in an agitated, surface-aerated bioreactor was examined. In the employed well-controlled bioreactors at high agitation rates, hybridoma cells in medium containing 1% fetal bovine serum rapidly die in the presence of a vortex with accompanying gas-bubble entrainment, whereas non-agitated control cultures grow normally in medium containing 1% serum. Serum levels greater than 5% counteract the detrimental hydrodynamic effects due to agitation and bubble entrainment. The protective effect is present after short-term (less than 1 h) exposure to 10% serum concentration, suggesting a protection mechanism which is, at least in part, of a physical nature. The apparent cell yields on glucose, lactate, and glutamine decreased with decreasing growth rate due to low serum concentrations. The results are incorporated into a simple model in which the apparent growth rate is the sum of an invariable growth rate and a changing death rate. © 1990.
引用
收藏
页码:57 / 69
页数:13
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