ENHANCED INDUCTION OF TISSUE-TYPE PLASMINOGEN-ACTIVATOR IN NORMAL HUMAN-CELLS COMPARED TO CANCER-PRONE CELLS FOLLOWING IONIZING-RADIATION

被引:17
作者
FUKUNAGA, N [1 ]
BURROWS, HL [1 ]
MEYERS, M [1 ]
SCHEA, RA [1 ]
BOOTHMAN, DA [1 ]
机构
[1] UNIV MICHIGAN,MED CTR,DEPT RADIAT ONCOL,DIV CANC BIOL,1331 E ANN ST,RM 4131,ANN ARBOR,MI 48109
来源
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS | 1992年 / 24卷 / 05期
关键词
IONIZING RADIATION; TISSUE-TYPE PLASMINOGEN ACTIVATOR; SOS RESPONSE; BLOOMS SYNDROME; ATAXIA TELANGIECTASIA; FANCONIS ANEMIA;
D O I
10.1016/0360-3016(92)90479-2
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Normal human fibroblast (i.e., GM2936B, GM2907A, and IMR-90) and cancer-prone human fibroblast (i.e., Fanconi's anemia, Bloom's syndrome, and Ataxia telangiectasia) cells demonstrated the induction of intracellular and extracellular levels of tissue-type plasminogen activator (t-PA) at 6 and 12 hr, respectively, following ionizing radiation. Induced t-PA enzymatic activities following ionizing radiation were blocked by actinomycin D treatments. t-PA enzymatic activities were induced over 14-fold in Ataxia telangiectasia cells, over 9-fold in Bloom's syndrome cells, and over 6-fold in Fanconi's anemia cells, as compared to normal human fibroblasts. Similarly, the induction of t-PA mRNA levels in cancer-prone cells were between 5- to 10-fold higher than those observed in normal cells following equitoxic doses of ionizing radiation. Temporal induction of t-PA mRNA levels for normal and cancer-prone human cells were consistent with quantifiable enzymatic activities. The elevated induction of an intracellular protease (i.e., t-PA) in cancer-prone human cells is reminiscent of an "SOS"-like response observed in yeast and bacteria.
引用
收藏
页码:949 / 957
页数:9
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