AN OCTAMER MOTIF CONTRIBUTES TO THE EXPRESSION OF THE RETINOIC ACID-REGULATED ZINC FINGER GENE REX-1 (ZFP-42) IN F9 TERATOCARCINOMA CELLS

被引:57
作者
HOSLER, BA
ROGERS, MB
KOZAK, CA
GUDAS, LJ
机构
[1] CORNELL UNIV, MED CTR, COLL MED, DEPT PHARMACOL, NEW YORK, NY 10021 USA
[2] HARVARD UNIV, SCH MED, BOSTON, MA 02115 USA
[3] NIAID, MOLEC MICROBIOL LAB, BETHESDA, MD 20892 USA
关键词
D O I
10.1128/MCB.13.5.2919
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The message for the zinc finger gene Rex-1 (Zfp-42) is expressed in undifferentiated murine F9 teratocarcinoma cells and embryonic stem cells. Expression of Rex-1 is reduced at the transcriptional level when F9 cells are induced by the addition of retinoic acid (RA) to differentiate. We have isolated genomic DNA for the Rex-1 gene (Zfp-42), characterized the gene's structure, and mapped the gene to mouse chromosome 8. Promoter elements contributing to the regulation of the Rex-1 promoter in F9 cells have been identified. A region required for Rex-1 promoter activity in F9 stem cells contains an octamer motif (ATTTGCAT) which is a binding site for octamer transcription factor members of the POU domain family of DNA-binding proteins. Rex-1 reporter plasmids including this octamer site also exhibited reduced expression in F9 cells treated with RA. Thus, the octamer motif is a regulatory element required for the activity of the Rex-1 promoter in F9 stem cells, and this motif contributes to the negative regulation by RA of the transcription of the Rex-1 gene. As an initial confirmation of the in vivo relevance of the isolated fragment, a larger Rex-1 promoter fragment, also containing the octamer site, was able to promote expression of the bacterial lacZ gene in mouse embryos at the morula stage.
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页码:2919 / 2928
页数:10
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