DETERMINATION OF CLODRONATE IN AQUEOUS-SOLUTIONS BY HPLC USING POSTCOLUMN DERIVATIZATION

被引：15

作者：

VIRTANEN, V

LAJUNEN, LHJ

机构：

[1] Department of Chemistry, University of Oulu, SF-90570 Oulu, Linnanmaa

来源：

TALANTA | 1993年 / 40卷 / 05期

关键词：

D O I：

10.1016/0039-9140(93)80275-V

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the determination of clodronate at ppm levels in aqueous solutions by HPLC is presented. Clodronate is first separated by anion exchange chromatography using a solution of nitric acid and sodium nitrate as eluent. Th-EDTA-XO mixed ligand complex is used as a postcolumn reagent for clodronate. Clodronate reacts quantitatively with this complex in slightly acid solutions, and a change in the absorbance of Th-EDTA-XO is used as a measure for the clodronate concentration. The detection limit is about 3 ppm, and the linear dynamic range of the method extends up to about 200 ppm. The RSD values varied between 5 and 16%. The effect of pH on appearance and retention of different forms of clodronate is studied. Different forms of clodronate can be separated and detected in the used anion-exchange system.

引用

页码：661 / 667

页数：7

共 20 条

[1] DETERMINATION OF (DICHLOROMETHYLENE)DIPHOSPHONATE IN PHYSIOLOGICAL FLUIDS BY ION-EXCHANGE CHROMATOGRAPHY WITH PHOSPHORUS-SELECTIVE DETECTION [J].