CHARACTERIZATION OF A PROSTATE CARCINOMA MUCIN-LIKE ANTIGEN (PMA)

被引:18
作者
BECKETT, ML
WRIGHT, GL
机构
[1] EASTERN VIRGINIA MED SCH,DEPT MICROBIOL & IMMUNOL,NORFOLK,VA 23501
[2] EASTERN VIRGINIA MED SCH,DEPT UROL,NORFOLK,VA 23501
[3] EASTERN VIRGINIA MED SCH,VIRGINIA PROSTATE CTR,NORFOLK,VA 23501
关键词
D O I
10.1002/ijc.2910620610
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
A recently identified, high m.w. human tumor antigen, reactive with monoclonal antibody (MAb) PD41 and designated prostate mucin antigen (PMA), was found to have expression highly restricted to prostate carcinomas. Both biochemical and immunological characteristics of this antigen and its relationship to other tumor-associated mucins and various species of submaxillary mucins were evaluated. Immunohistochemical examination of submaxillary tissues revealed differential expression of this antigen and other human tumor-associated mucins, with MAb, PD41 expression found only in bovine submaxillary gland serous cells. Neuraminidase treatment enhanced antibody binding by 50%, suggesting partial masking of the PD41 epitope by sialic acid. Antigenicity was reduced by treatment with alkaline-borohydride, sodium metaperiodate, beta-galactosidase, O-glycanase, and various proteolytic enzymes. MAb PD41 antibody binding also could be significantly reduced by selected lectins and sugars suggesting that: the immunodominant carbohydrate involved in the epitope was an O-linked oligosaccharide containing N-acetyl galactosamine as a major component. This antigen was further distinguished from T, Tn, or Sialyl-Tn antigens and blood group carbohydrate antigens by radioimmunometric analyses. Cross-blocking and double determinant WA experiments also showed a distinction between the PD41 epitope and several well-characterized tumor-associated mucin antigens such as MUC1, CEA, M344, OC125, and AR3 as well as bovine submaxillary core protein. Our results indicate that the PD41-reactive epitope is a non-acidic O-linked carbohydrate or glycopeptide epitope with restricted expression in prostate carcinomas and bovine submaxillary glands. This expression is distinct from other mucin-like tumor-associated antigens identified in human prostate carcinomas. (C) 1995 Wiley-Liss, Inc.
引用
收藏
页码:703 / 710
页数:8
相关论文
共 22 条
[1]  
ABE M, 1989, CANCER RES, V49, P2834
[2]  
BECKETT ML, 1991, CANCER RES, V51, P1326
[3]  
BECKETT ML, 1992, 2ND INT WORKSH CARC
[4]   CLONING AND CDNA SEQUENCE OF A BOVINE SUBMAXILLARY-GLAND MUCIN-LIKE PROTEIN CONTAINING 2 DISTINCT DOMAINS [J].
BHARGAVA, AK ;
WOITACH, JT ;
DAVIDSON, EA ;
BHAVANANDAN, VP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (17) :6798-6802
[5]  
BOBEK LA, 1993, J BIOL CHEM, V268
[6]  
BURCHELL J, 1983, J IMMUNOL, V131, P508
[7]   ISOLATION AND CHARACTERIZATION OF HUMAN CERVICAL-MUCUS GLYCOPROTEINS [J].
CARLSTEDT, I ;
LINDGREN, H ;
SHEEHAN, JK ;
ULMSTEN, U ;
WINGERUP, L .
BIOCHEMICAL JOURNAL, 1983, 211 (01) :13-22
[8]   A SPECTRUM OF MONOCLONAL-ANTIBODIES REACTIVE WITH HUMAN MAMMARY-TUMOR CELLS [J].
COLCHER, D ;
HAND, PH ;
NUTI, M ;
SCHLOM, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (05) :3199-3203
[9]  
DAHIYA R, 1993, CANCER RES, V53, P1437
[10]  
Feller W F, 1990, Immunol Ser, V53, P631