A CONVENIENT VOID VOLUME MARKER FOR SEVERAL CHIRAL HPLC COLUMNS

被引：93

作者：

PIRKLE, WH

WELCH, CJ

机构：

[1] School of Chemical Sciences, University of Illinois, Urbana, Illinois

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1991年 / 14卷 / 01期

关键词：

D O I：

10.1080/01483919108049593

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparison of a number of potential void volume markers under normal phase conditions shows 1,3,5-Tri-t-butylbenzene (TTBB) to be essentially unretained on three commercially available chiral stationary phases (CSPs). Elution times observed for TTBB agree well with void times determined by the method of minor disturbance, or with the pycnometrically determined total mobile phase volume (V(m)). Therefore TTBB is recommended for use as a void volume marker for normal phase separations with these CSPs.

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页码：1 / 8

页数：8

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