MICROTUBULE ORGANIZATION AND CELL-DIVISION IN EMBRYOGENIC PROTOPLAST CULTURES OF WHITE SPRUCE (PICEA-GLAUCA)

被引：23

作者：

FOWKE, LC ^{[1
]}

ATTREE, SM ^{[1
]}

WANG, H ^{[1
]}

DUNSTAN, DI ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA,INST PLANT BIOTECHNOL,SASKATOON S7N 0W9,SASKATCHEWAN,CANADA

来源：

PROTOPLASMA | 1990年 / 158卷 / 1-2期

关键词：

Cell division; Microtubules; Picea glauca; Protoplasts; Somatic embryos;

D O I：

10.1007/BF01323277

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Immunofluorescence methods were developed for examining the distribution of microtubules in freshly isolated and cultured protoplasts and regenerated somatic embryos of white spruce (Picea glauca). Freshly isolated protoplasts consisted of both uniand multinucleate types. Uninucleate protoplasts established parallel cortical microtubules during cell wall formation and cell shaping, divided within 24 h and developed into somatic embryos in culture. Dividing cells were characterized by preprophase bands (PPBs) of microtubules, atypical spindle microtubules focused at the poles and a typical phragmoplast at telophase. Multinucleate protoplasts also established parallel arrays of cortical microtubules during cell wall formation. In addition their nuclei divided synchronously within 4 days, then cell walls formed between the daughter nuclei. Individual multinucleate protoplast-derived colonies subsequently gave rise to elongate suspensor cells thereby forming embryo-like structures by 7 days. © 1990 Springer-Verlag.

引用

页码：86 / 94

页数：9

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