CLONING AND MOLECULAR CHARACTERIZATION OF THE MURINE MACROPHAGE 68-KDA PROTEIN-KINASE-C SUBSTRATE AND ITS REGULATION BY BACTERIAL LIPOPOLYSACCHARIDE

被引：92

作者：

SEYKORA, JT ^{[1
]}

RAVETCH, JV ^{[1
]}

ADEREM, A ^{[1
]}

机构：

[1] MEM SLOAN KETTERING CANC CTR,PROGRAM MOLEC BIOL,DEWITT WALLACE RES LAB,NEW YORK,NY 10021

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 06期

关键词：

MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE; MYRISTOYLATION; PHOSPHORYLATION; SIGNAL TRANSDUCTION; ACTIN;

D O I：

10.1073/pnas.88.6.2505

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have isolated and characterized a cDNA clone encoding the murine macrophage 68-kDa protein kinase C substrate, which is homologous to the 80- to 87-kDa protein identified by the acronym MARCKS (myristoylated alanine-rich C kinase substrate). The murine MARCKS cDNA clone encodes an acidic protein of 309 amino acids with a calculated molecular weight of 29,661. Transfection of the murine MARCKS gene into TK-L fibroblasts produced a myristoylated protein kinase C substrate that migrated on SDS/PAGE with an apparent molecular mass of 68 kDa. Peptide mapping studies indicated that MARCKS produced by the transfected gene was indistinguishable from the endogenous murine macrophage protein. Comparison of the murine macrophage sequence with the previously published chicken and bovine brain sequences revealed two conserved domains: an N-terminal membrane-binding domain and a phosphorylation domain that also contains calmodulin and actin binding sites. In murine peritoneal macrophages, bacterial lipopolysaccharide increased MARCKS mRNA levels by > 30-fold. Multiple MARCKS transcripts were observed and could be accounted for by differential polyadenylylation and incomplete processing. Genomic Southern blot analysis suggested a single MARCKS gene per haploid genome.

引用

页码：2505 / 2509

页数：5

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