A SOLUBLE CELLULAR FACTOR DIRECTLY STIMULATES CA2+ ENTRY IN NEUTROPHILS

被引：39

作者：

DAVIES, EV

HALLETT, MB

机构：

[1] Univ Wales Coll Med, Dept Surg, S Glam, Wales, Molec Signalling Grp

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 206卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.1048

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A soluble factor extracted from neutrophils and P388D1 cells, stimulated a transient rise in cytosolic free Ca2+ and a small increase in the permeability to Mn2+ in fura2-loaded neutrophils. These effects were not prevented by blockade of formylated peptide receptors by t-boc-met-leu-phe. The rise in cytosolic free Ca2+ was partly attributed to transmembrane influx and partly due to store release. Ca2+ store release but not transmembrane influx was inhibited by the PLC inhibitor, U73122, demonstrating a direct effect of the factor on channel opening. It was concluded that the soluble cellular factor directly stimulated Ca2+ entry in neutrophils. (C) 1995 Academic Press, Inc.

引用

页码：348 / 354

页数：7

共 17 条

[1] THE USE OF FURA-2 TO DETERMINE THE RELATIONSHIP BETWEEN CYTOPLASMIC FREE CA-2+ AND OXIDASE ACTIVATION IN RAT NEUTROPHILS [J].