ISOLATION OF A CDNA FOR THE CATALYTIC SUBUNIT OF RAT-LIVER GLUCOSE-6-PHOSPHATASE - REGULATION OF GENE-EXPRESSION IN FAO HEPATOMA-CELLS BY INSULIN, DEXAMETHASONE AND CAMP

被引：119

作者：

LANGE, AJ ^{[1
]}

ARGAUD, D ^{[1
]}

ELMAGHRABI, MR ^{[1
]}

PAN, WS ^{[1
]}

MAITRA, SR ^{[1
]}

PILKIS, SJ ^{[1
]}

机构：

[1] SUNY STONY BROOK,HLTH SCI CTR,DEPT EMERGENCY MED & SURG,STONY BROOK,NY 11794

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 201卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.1702

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

cDNA clones coding for the catalytic subunit of rat liver glucose-6-phosphatase (EC 3.1.3.9) were isolated from a rat liver cDNA library in lambda gt(11) phage. The sequence of the cDNA and the amino acid sequence derived from it were greater than 90% identical to the corresponding sequences for the mouse and human forms of liver glucose-6-phosphatase. Northern blot analysis of RNA from FAO hepatoma cells revealed that dexamethasone induced the glucose-6-phosphatase mRNA while insulin suppressed its expression. When both hormones were added together insulin completely suppressed the effect of glucocorticoid. cAMP addition alone decreased the abundance of glucose-6-phosphatase mRNA. The results demonstrate multihormonal regulation of gene expression of hepatic glucose-6-phosphatase and support a dominant role for insulin. (C) 1994 Academic Press, Inc.

引用

页码：302 / 309

页数：8

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