CONSTRUCTION OF BRANCHED DNA (BDNA) MOLECULES BY CHEMICAL LIGATION

被引：7

作者：

DOLINNAYA, N

GRYAZNOV, S

AHLE, D

CHANG, CA

SHABAROVA, ZA

URDEA, MS

HORN, T

机构：

[1] NORTHWESTERN UNIV, DEPT CHEM, EVANSTON, IL 60208 USA

[2] MOSCOW MV LOMONOSOV STATE UNIV, DEPT CHEM, MOSCOW 119899, RUSSIA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1994年 / 4卷 / 08期

关键词：

D O I：

10.1016/S0960-894X(01)80672-7

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Chemical ligation has been investigated in the assembly of branched DNA (bDNA) molecules. Water soluble coupling reagents were used to ligate single-stranded linear oligomers to bDNA molecules containing 5 and 15 branches. The ligations were particularly efficient when one of the oligomers was partially double-stranded constructed by branching to create a terminal ligation linker (AutoLink). Branched DNA molecules containing more than 1000 nucleotides were assembled.

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页码：1011 / 1018

页数：8

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