A MORPHOLOGICAL-STUDY OF UNFERTILIZED OOCYTES AND ABNORMAL EMBRYOS IN HUMAN INVITRO FERTILIZATION

被引:26
作者
BALAKIER, H
CASPER, RF
机构
[1] Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Toronto, Toronto, Ontario
来源
JOURNAL OF IN VITRO FERTILIZATION AND EMBRYO TRANSFER | 1991年 / 8卷 / 02期
关键词
AGED OOCYTES; SPONTANEOUS ACTIVATION; ABNORMAL EMBRYOS; POLYSPERMY; HUMAN INVITRO FERTILIZATION (IVF);
D O I
10.1007/BF01138658
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
The morphology of human, unfertilized oocytes and abnormal embryos cultured in vitro for 48-72 hr was examined in an attempt to learn more about oocyte maturation and reproductive failure in in vitro fertilization (IVF). About 21% of the unfertilized oocytes were totally degenerated. The majority (56%) of the remaining oocytes was arrested at the metaphase II stage. They contained coherent chromosomal plates and had extruded the first polar body with nuclear material. About 13% of oocytes underwent spontaneous activation. In most of these cases the second polar body was retained and many subnuclei or one big nucleus was formed. Five percent of metaphase II oocytes penetrated by sperm were not activated, likely as a result of oocyte immaturity. The developmental ability of abnormal embryos was poor. Several one-cell-stage zygotes were arrested at the pronuclear stage or at mitosis of the first mitotic division. Polyspermic embryos, especially those which contained four or more pronuclei, did not divide or formed uneven, multinucleated blastomeres. However, some triploid and tetraploid embryos often appeared normal morphologically despite their lethal chromosomal abnormalities.
引用
收藏
页码:73 / 79
页数:7
相关论文
共 28 条
  • [1] Edwards R.G., Causes of early embryonic loss in human pregnancy, Hum Reprod, 1, pp. 185-198, (1986)
  • [2] Sathananthan A.H., Trounson A.O., The human pronuclear ovum: Fine structure of monospermic and polyspermic fortilization in vitro, Gamete Res, 12, pp. 385-398, (1985)
  • [3] Van Blerkom J., Henry G., Cytogenetic analysis of living human eggs: cellular basis and developmental consequences of perturbations in chromosomal organization and complement, Hum Repord, 3, pp. 777-790, (1988)
  • [4] Ma S., Kalousek D.K., Zouves C., Yuen B.H., Gomel V., Moon Y.S., Chromosome analysis of human eggs failing to fertilize in vitro, Fertil Steril, 51, pp. 992-997, (1989)
  • [5] Gonen Y., Jacobson W., Casper R.F., Gonadotropin suppresion with oral contraceptives before in vitro fertilization, Fertil Steril, 53, pp. 282-287, (1990)
  • [6] Quinn P., Warnes G.M., Kerin J.F., Kirby C., Culture factors in relation to the success of human in vitro fertilization and embryo transfer, Fertil Steril, 41, pp. 202-209, (1984)
  • [7] Veeck L.L., Atlas of the Human Oocyte and Early Conceptus, (1986)
  • [8] Tarkowski A.K., Development of single blastomeres, Methods in Mammalian Embryology, pp. 172-185, (1971)
  • [9] Clarke H.J., Masui Y., Inhibition by dibutyryl cyclic AMP of the transition to metaphase of mouse oocyte nuclei and its reversal by cell fusion to metaphase oocytes, Dev Biol, 108, pp. 32-37, (1985)
  • [10] Tarkowski A.K., An air-drying method for chromosome preparations from mouse eggs, Cytogenetics, 4, pp. 394-400, (1966)