HEMODIALYSIS MEMBRANE BIOCOMPATIBILITY - THE CASE OF ERYTHROPOIETIN

被引：21

作者：

CHEUNG, AK

HOHNHOLT, M

LEYPOLDT, JK

DESPAIN, M

机构：

[1] Medical Service and Research Service, Veterans Affairs Medical Center, Division of Nephropolgy, Department of Medicine, University of Utah School of Medicine, Salt Lake, UT

来源：

BLOOD PURIFICATION | 1991年 / 9卷 / 03期

关键词：

HEMODIALYSIS; MEMBRANE; ERYTHROPOIETIN; PROTEIN; ADSORPTION;

D O I：

10.1159/000170012

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Protein adsorption occurs upon blood exposure to hemodialysis membranes. While adsorption of certain proteins may be beneficial, adsorption of others may be undesirable. In the present study, we investigated the binding of recombinant human erythropoietin (rHuEPO) to different types of hemodialysis membranes in vitro. The amount of rHuEPO bound was dependent on the time of incubation, the concentration of the protein offered and the membrane material. Membranes made from the copolymer of polyacrylonitrile and methallyl sulfonate (AN69) bound the greatest quantity of rHuEPO. The dose-response curve of the binding to this type of membrane was linear. At the highest concentration examined, AN69 membranes bound 30 times more rHuEPO than did Cuprophan. This amount bound to AN69 was also greater than that which is expected to be present in a monolayer covering the nominal membrane surface area. We conclude that, under certain conditions, the binding of rHuEPO can be substantial and represents an example in which protein binding to hemodialysis membrane may be disadvantageous.

引用

页码：153 / 163

页数：11

共 26 条

[1]

Hallgren R., Venge P., Danielson B.G., Neutrophil and eosinophil degranulation during hemodialysis are mediated by the dialysis membrane, Nephron, 32, pp. 329-334, (1982)

[2]

Haeffher-Cavaillon N., Cavaillon J.-M., Ciancioni C., Et al., In vivo induction of interleukin-1 during hemodialysis, Kidney Int, 35, pp. 1212-1218, (1989)

[3]

Berger S., Salzman E.W., Thromboembolic complications of prosthetic devices, Prog Hemosl Thromb, 2, pp. 273-309, (1974)

[4]

Goldman M., Dhaene M., Vanherweghem J.-L., Removal of p:-microglobulin by adsorption on dialysis membranes, Nephrol Dial Transplant, 2, pp. 576-578, (1987)

[5]

Chenoweth D.E., Cheung A.K., Henderson L.W., Anaphylatoxin formation during hemodialysis: Effects of different dialyzer membranes, Kidney Int, 24, pp. 764-769, (1983)

[6]

Cheung D.E., Ward A.K.D.M., Et al., Anaphylatoxin formation during hemodialysis: Comparison of new and re-used dialyzers, Kidney Int, 24, pp. 770-774, (1983)

[7]

Hakim R.M., Fearon D.T., Lazarus J.M., Biocom-patibility of dialysis membranes: Effects of chronic complement activation, Kidney Int, 26, pp. 194-200, (1984)

[8]

Eschbach J.W., Egrie J.C., Downing M.R., Et al., Correction of the anemia of end-stage renal disease with recombinant human erythropoietin: Results of a combined phase I and II clinical trial, N Engl J Med, 316, pp. 73-78, (1987)

[9]

Cheung A.K., Parker C.I., Wilcox L.W., Janatova J., Activation of complement by hemodialysis membranes: Polyacrylonitrile binds more C3a than cu-prophan, Kidney Int, 37, pp. 1055-1059, (1990)

[10]

Matthews D.E., Farewell V.T., Using and Understanding Medical Statistics, (1985)

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