DIFFERENTIAL EXPRESSION OF KERATAN SULFATE PROTEOGLYCANS FIBROMODULIN, LUMICAN AND AGGRECAN IN NORMAL AND FIBROTIC RAT-LIVER

被引:35
作者
KRULL, NB
GRESSNER, AM
机构
[1] UNIV MARBURG,DEPT CLIN CHEM,W-3550 MARBURG,GERMANY
[2] UNIV MARBURG,CENT LAB,W-3550 MARBURG,GERMANY
关键词
KERATAN SULFATE; PROTEOGLYCANS; LUMICAN; FIBROMODULIN; AGGRECAN; EXPRESSION;
D O I
10.1016/0014-5793(92)81407-D
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study we investigated in rat liver the expression of genes coding for the core proteins of fibromodulin, lumican and aggrecan. By means of Northern analysis and in situ hybridization we present evidence for their differential transcription during liver fibrogenesis. Whereas no fibromodulin expression could been detected, both lumican and aggrecan transcripts were found displaying different time-courses of expression during the fibrogenic process. Based on studies performed in non-hepatic tissues, these proteoglycans are considered to have keratan sulphate glycosaminoglycan side chains. The expression of the respective core protein genes in liver is unexpected since published data have shown neither keratan sulphate nor its synthesis de novo in this tissue. The results also point to a putative role of aggrecan in the modulation of the inflammatory process in the liver.
引用
收藏
页码:47 / 52
页数:6
相关论文
共 23 条
[1]   FORMATION OF EXTRACELLULAR-MATRIX IN NORMAL RAT-LIVER - LIPOCYTES AS A MAJOR SOURCE OF PROTEOGLYCAN [J].
ARENSON, DM ;
FRIEDMAN, SL ;
BISSELL, DM .
GASTROENTEROLOGY, 1988, 95 (02) :441-447
[2]  
BLOCHBERGER TC, 1992, J BIOL CHEM, V267, P347
[3]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[4]  
CHURCH GM, 1984, P NATL ACAD SCI USA, V81, P1191
[5]   IMMUNOENZYMATIC LABELING OF MONOCLONAL-ANTIBODIES USING IMMUNE-COMPLEXES OF ALKALINE-PHOSPHATASE AND MONOCLONAL ANTI-ALKALINE PHOSPHATASE (APAAP COMPLEXES) [J].
CORDELL, JL ;
FALINI, B ;
ERBER, WN ;
GHOSH, AK ;
ABDULAZIZ, Z ;
MACDONALD, S ;
PULFORD, KAF ;
STEIN, H ;
MASON, DY .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1984, 32 (02) :219-229
[6]  
DELBRUCK A, 1968, BIOCHEM, V6, P460
[7]  
DOEGE K, 1987, J BIOL CHEM, V262, P17757
[8]  
DRICKAMER K, 1988, J BIOL CHEM, V263, P9557
[9]  
FISHER LW, 1991, J BIOL CHEM, V266, P14371
[10]  
FISHER LW, 1987, J BIOL CHEM, V262, P9702