INHIBITORY EFFECTS OF PECTIC SUBSTANCES ON ACTIVATED HYALURONIDASE AND HISTAMINE-RELEASE FROM MAST-CELLS

被引：42

作者：

SAWABE, Y

NAKAGOMI, K

IWAGAMI, S

SUZUKI, S

NAKAZAWA, H

机构：

[1] FERMENTAT RES INST,TSUKUBA,JAPAN

[2] NATL INST PUBL HLTH,TOKYO,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1137卷 / 03期

关键词：

PECTIC SUBSTANCE; ANTIHYALURONIDASE ACTIVITY; ANTIHISTAMINE-RELEASE ACTIVITY; ANTIALLERGIC ACTIVITY; (GYMNEMA-SYLVESTRE);

D O I：

10.1016/0167-4889(92)90147-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper, we report the effect of pectic substances and D-galacturonic acid, the main constituent of pectic substances, on activated hyaluronidase and histamine release from mast cells. Although D-galacturonic acid itself showed no inhibition, IC50 values of hyaluronidase inhibition were correlated with the D-galacturonic-acid content of pectic substances. It was thought that the polymerization of D-galacturonic acid was necessary for inhibition of activated hyaluronidase. This type of inhibition was suggested to be non-competitive by the Lineweaver-Burk plot. Furthermore, pectic substances, including those purified from Gymnema sylvestre, inhibited histamine release from isolated rat peritoneal mast cells, which had been induced by the antigen. These results suggest that pectic substances may have anti-allergic activities.

引用

页码：274 / 278

页数：5

共 12 条

[1]

ARONSON NN, 1967, J BIOL CHEM, V242, P437

[2]

BEFUS AD, 1982, J IMMUNOL, V128, P2475

[3] A MODIFIED URONIC ACID CARBAZOLE REACTION [J].