TANDEM MASS-SPECTROMETRY OF PEPTIDES - SEQUENCE INFORMATION-BASED ON NEUTRAL LOSSES .1. ISOMERIC DIPEPTIDES

被引：19

作者：

CORDERO, MM

WESDEMIOTIS, C

机构：

[1] Department of Chemistry, University of Akron, Akron, Ohio

来源：

ORGANIC MASS SPECTROMETRY | 1993年 / 28卷 / 10期

关键词：

D O I：

10.1002/oms.1210281011

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The neutral losses arising on the collisionally activated dissociation (CAD) of protonated dipeptides (MH+) are reionized by collision and detected in neutral fragment-reionization (N(f)R) mass spectra. For the dipeptides investigated, which encompass Ala-Gly/Gly-Ala, Asp-Gly/Gly-Asp, Phe-Gly/Gly-Phe and Trp-Ala/Ala-Trp, the N(f)R spectra of each pair of isomers are distinct and permit the identification of the correct C-terminal amino acid. The precise structure of this amino acid is elucidated by comparison of the N(f)R spectra with reference collision-induced dissociative ionization (CIDI) mass spectra of authentic Gly, Ala, Asp, Phe and Trp, acquired from beams of these amino acids formed by dissociation of their proton-bound dimers.

引用

页码：1041 / 1046

页数：6

共 19 条

[11] ANGULAR-DEPENDENCE OF INTERNAL ENERGY-DISTRIBUTIONS OF ACTIVATED FE(CO)5.+ AND W(CO)6.+ IONS - THE CONTRIBUTIONS OF VIBRATIONAL AND ELECTRONIC EXCITATION MECHANISMS IN KILOELECTRON VOLT COLLISIONS [J].