PRODUCTION AND IN-VITRO EVALUATION OF GELATIN MICROSPHERES CONTAINING AN ANTITUMOR TETRA-AMIDINE

被引：26

作者：

NASTRUZZI, C

PASTESINI, C

CORTESI, R

ESPOSITO, E

GAMBARI, R

MENEGATTI, E

机构：

[1] Department of Pharmaceutical Science, University of Ferrara

[2] Biotechnology Centre, University of Ferrara

来源：

JOURNAL OF MICROENCAPSULATION | 1994年 / 11卷 / 03期

关键词：

D O I：

10.3109/02652049409040454

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The preparation and characterization of gelatin microspheres (GMs) containing the antitumour aromatic tetra-amidine TAPP-Br and its release characteristics from microspheres are described. Spherical gelatin microspheres, showing a high percentage of drug encapsulation (over 95 per cent) and an average diameter of 16 mum were obtained by a coacervation. method. In addition, the effects of various reaction parameters on the production and chemical hardening of GMs have been investigated. In order to compare the TAPP-Br release profiles from GMs prepared with different procedures, in vitro studies were carried out. The biological effect of TAPP-Br released from gelatin microspheres was determined on 'in vitro' cultured tumour cell lines, showing that, after encapsulation, TAPP-Br still retains antiproliferative activity comparable with that exhibited by the free drug. Taken together, the results obtained suggest that gelatin-based microspheres offer excellent potential as carriers for the controlled release of polyamidines in anticancer chemotherapy.

引用

页码：249 / 260

页数：12

共 22 条

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