ELECTROGENIC AND PHOSPHATIDYLINOSITOL-4-MONOPHOSPHATE-STIMULATED CL- TRANSPORT BY CL- PUMP IN THE RAT-BRAIN

被引：27

作者：

ZENG, XT ^{[1
]}

HARA, M ^{[1
]}

INAGAKI, C ^{[1
]}

机构：

[1] KANSAI MED UNIV,DEPT PHARMACOL,MORIGUCHI,OSAKA 570,JAPAN

来源：

BRAIN RESEARCH | 1994年 / 641卷 / 01期

关键词：

CL-; PUMP; TRANSPORT; ELECTROGENIC; PHOSPHATIDYLINOSITOL-4-MONOPHOSPHATE; -ATPASE; RECONSTITUTION;

D O I：

10.1016/0006-8993(94)91833-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Rat brain Cl--ATPase was solubilized and reconstituted in asolectin liposomes. Phosphatidylinositol-4-monophosphate increased Cl--ATPase and ATP-dependent Cl- uptake activities in proteoliposomes. The ATP-dependent Cl- uptake was inhibited by a Cl--ATPase inhibitor, ethacrynic acid, and increased at an inside-positive membrane potential or in the presence of a protonophore. These findings suggest that Cl--ATPase is an electrogenic Cl- transporter, or a primary Cl- pump, probably regulated by phosphoinositide turnover in vivo.

引用

页码：167 / 170

页数：4

共 12 条

[1] THE CALCIUM PUMPING ATPASE OF THE PLASMA-MEMBRANE [J].

CARAFOLI, E .

ANNUAL REVIEW OF PHYSIOLOGY, 1991, 53 :531-547

[2] IS THERE A CL- PUMP [J].

GERENCSER, GA ;

WHITE, JF ;

GRADMANN, D ;

BONTING, SL .

AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 255 (05) :R677-R692

[3] EFFECTS OF SEVERAL ANIONS ON ETHACRYNIC-ACID HIGH-SENSITIVE AND LOW-SENSITIVE MG-ATPASE ACTIVITIES IN MICROSOMAL FRACTIONS FROM RABBIT CORTICAL GRAY-MATTER [J].

HARA, M ;

MIWA, S ;

FUJIWARA, M ;

INAGAKI, C .

BIOCHEMICAL PHARMACOLOGY, 1982, 31 (05) :877-879

[4] A CL--TRANSLOCATING ADENOSINE-TRIPHOSPHATASE IN ACETABULARIA-ACETABULUM .2. RECONSTITUTION OF THE ENZYME INTO LIPOSOMES AND EFFECT OF NET CHARGES OF LIPOSOMES ON CHLORIDE PERMEABILITY AND RECONSTITUTION [J].

IKEDA, M ;

OESTERHELT, D .

BIOCHEMISTRY, 1990, 29 (08) :2065-2070

[5] ATP-DEPENDENT C1- UPTAKE BY PLASMA-MEMBRANE VESICLES FROM THE RAT-BRAIN [J].

INAGAKI, C ;

SHIROYA, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 154 (01) :108-112

[6]

INAGAKI C, 1985, BIOCHEM PHARMACOL, V34, P175

[7]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[8]

MCCORMICK JI, 1985, J BIOL CHEM, V260, P5706

[9]

SHIROYA T, 1989, J BIOL CHEM, V264, P17416

[10] ATP-DEPENDENT AND DCCD-INSENSITIVE CL- UPTAKE BY MEMBRANE-VESICLES FROM THE RAT-BRAIN PLASMA-MEMBRANE FRACTIONS [J].

SHIROYA, T ;

CRAGOE, EJ ;

INAGAKI, C .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 160 (02) :469-473

← 1 2 →