FLUX-DEPENDENT INCREASE IN THE STOICHIOMETRY OF CHARGE TRANSLOCATION BY MITOCHONDRIAL ATPASE ATP SYNTHASE INDUCED BY ALMITRINE

被引：31

作者：

RIGOULET, M ^{[1
]}

FRAISSE, L ^{[1
]}

OUHABI, R ^{[1
]}

GUERIN, B ^{[1
]}

FONTAINE, E ^{[1
]}

LEVERVE, X ^{[1
]}

机构：

[1] FAC MED GRENOBLE, THERAPEUT & ETUD METAB LAB, LA TRONCHE, FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1018卷 / 01期

关键词：

(Beef heart); (Rat liver); Almitrine; ATP synthase; ATPase; Mitochondrion; Proton pump stoichiometry;

D O I：

10.1016/0005-2728(90)90114-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

After studying the effects of almitrine, a new kind of ATPase/ATP synthase inhibitor, on two kinds of isolated mammalian mitochondrion, we have observed that: (1) Almitrine inhibits oligomycin-sensitive ATPase; it decreases the ATP/O value of oxidative phosphorylations without any change in the magnitude of Δ \ ̃gmH. (2) Almitrine increases the mechanistic H+/ATP stoichiometry of ATPase as shown by measuring either (i) the extent of potassium acetate and of potassium phosphate accumulation sustained by ATP utilisation, or (ii) the electrical charge/ATP ( K+ ATP) ratio at steady-state of ATPase activity. (3) Rat liver mitochondria are at least 10-times more sensitive to almitrine than beef heart mitochondria. (4) The change in H+/ATP stoichiometry induced by almitrine depends on the magnitude of the flux through ATPase. The inhibitory effect of almitrine on ATPase/ATP synthase complex, as a consequence of such an H+/ATP stoichiometry change, is discussed. © 1990.

引用

页码：91 / 97

页数：7

共 22 条

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