EVIDENCE FOR ALTERNATIVE RNA SPLICING AND POSSIBLE ALTERNATIVE PROMOTERS IN THE HUMAN MUSCLE PHOSPHOFRUCTOKINASE GENE AT THE 5' UNTRANSLATED REGION

被引：30

作者：

NAKAJIMA, H ^{[1
]}

YAMASAKI, T ^{[1
]}

NOGUCHI, T ^{[1
]}

TANAKA, T ^{[1
]}

KONO, N ^{[1
]}

TARUI, S ^{[1
]}

机构：

[1] OSAKA UNIV,SCH MED,DEPT NUTR & PHYSIOL CHEM,OSAKA 553,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 166卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)90856-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three mRNA species for human muscle phosphofructokinase containing heterogeneous 5′ untranslated sequences were identified through cDNA cloning. Type A mRNA was essentially the same as that reported previously (Nakajima, H., et al. (1987) FEBS Lett. 223, 113). Type B mRNA was considered to be the major gene product, which contained an extra non-coding sequence within the 5′ untranslated region of type A mRNA. Amplification of mRNA by polymerase chain reaction revealed that types A and B mRNAs shared a common precursor RNA, and were alternatively spliced. Type C mRNA, homologous to the cDNA sequence from a placenta library (Sharma, P. M. et al., (1989) Gene 77, 177), was considered to be under the control of an alternative promotor. © 1990.

引用

页码：637 / 641

页数：5

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