MINI-PERCOLL - A NEW METHOD OF SEMEN PREPARATION FOR IVF IN SEVERE MALE FACTOR INFERTILITY

被引：149

作者：

ORD, T

PATRIZIO, P

MARELLO, E

BALMACEDA, JP

ASCH, RH

机构：

[1] Department of Obstetrics and Gynecology, University of California, Irvine (UCI), Orange

来源：

HUMAN REPRODUCTION | 1990年 / 5卷 / 08期

关键词：

DISCONTINUOUS PERCOLL GRADIENT; MALE FACTOR; OLIGOASTHENOZOOSPERMIA; RESUSPENSION;

D O I：

10.1093/oxfordjournals.humrep.a137233

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Conventional methods of semen preparation of IVF, such as swim-up sedimentation, regular Percoll gradients, are not very effective in the treatment of severe oligoasthenozoospermia (total motile count < 5 x 10(6)). For these cases, a new method of sperm preparation consisting of a reduced volume of a discontinuous Percoll gradient (mini-Percoll) has been utilized. The results obtained were analysed by comparing mini-Percoll versus resuspension in two groups of patients with severe male factor infertility. In the mini-Percoll group, a statistically significant improvement (P < 0.001) was obtained in the post-treatment seminal parameters of motility, progression and proportion of normal forms. In the resuspension group (n = 12), only eight of 108 oocytes inseminated were fertilized (7%), while in the mini-Percoll group (n = 29), 124 out of 336 oocytes were fertilized (40%) (P < 0.001). Furthermore, in the mini-Percoll group, a higher pregnancy rate (25 versus 7%) and low abortion rate (22 versus 33%) were observed. Although more studies are necessary to confirm these preliminary data, mini-Percoll seems to be a valid laboratory tool for semen samples of extremely poor quality.

引用

页码：987 / 989

页数：3

共 13 条

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