COMBINED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC CONTINUOUS-FLOW FAST ATOM BOMBARDMENT MASS-SPECTROMETRIC ANALYSIS OF ACYLCOENZYME-A COMPOUNDS

被引：23

作者：

NORWOOD, DL

BUS, CA

MILLINGTON, DS

机构：

[1] Division of Pediatric Genetics and Metabolism, Duke University Medical Center, Durham, NC 27710

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 527卷 / 02期

关键词：

D O I：

10.1016/S0378-4347(00)82113-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the analysis of coenzyme A thioesters which employs continuous-flow fast atom bombardment mass spectrometric detection is presented. The chromatographic system utilizes gradient elution with reversed-phase conditions using ammonium acetate-acetonitrile from both standard analytical (3.9 mm I.D.) and microbore (1 mm I.D.) columns. Applications to coenzyme A thioesters of various acyl group chain length (C2-C18) and functionality (-COOH, -OH, -CC-) are described. The system is also applied to an in vitro enzyme reaction (crotonase) to directly follow the disappearance of substrate and appearance of product. The mass spectrometry of coenzyme A thioesters, their chromatographic behavior, system stability, and sensitivity of detection are discussed. © 1990.

引用

页码：289 / 301

页数：13

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