GROUP-II PHOSPHOLIPASE-A2 INHIBITORS SUPPRESSED LYSOPHOSPHATIDYLSERINE-DEPENDENT DEGRANULATION OF RAT PERITONEAL MAST-CELLS

被引：48

作者：

MURAKAMI, M ^{[1
]}

KUDO, I ^{[1
]}

FUJIMORI, Y ^{[1
]}

SUGA, H ^{[1
]}

INOUE, K ^{[1
]}

机构：

[1] UNIV TOKYO,FAC PHARMACEUT SCI,DEPT HLTH CHEM,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 181卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91249-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rat peritoneal mast cells were sensitized with IgE and challenged with the specific antigen in the presence of lysophosphatidylserine (lysoPS), an essential co-factor for rodent connective tissue mast cell degranulation, and the effects of phospholipase A2 inhibitors were examined. Mepacrine, a known inhibitor of phospholipase A2, at concentrations below 10-5 M and anti-rat 14-kDa group II phospholipase A2 antibody inhibited histamine release, while they did not affect the prostaglandin generation. Like histamine release, prostaglandin generation in IgE- and antigen- challenged rat peritoneal mast cells was dependent on the presence of lysoPS. These results indicate that 14-kDa group II phospholipase A2 may play an essential role in IgE-, antigen-, and lysoPS-dependent degranulation process of rat peritoneal mast cells and that the mechanism whereby it participates may not be due to the production of lysoPS from PS in mast cell membranes. © 1991.

引用

页码：714 / 721

页数：8

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