PREFERENTIAL MODIFICATION OF GUANINE BASES IN DNA BY DIMETHYLDIOXIRANE AND ITS APPLICATION TO DNA SEQUENCING

被引：5

作者：

DAVIES, RJH ^{[1
]}

BOYD, DR ^{[1
]}

KUMAR, S ^{[1
]}

SHARMA, ND ^{[1
]}

STEVENSON, C ^{[1
]}

机构：

[1] QUEENS UNIV BELFAST, SCH CHEM, BELFAST BT9 7BL, ANTRIM, NORTH IRELAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 169卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91437-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

From gel sequencing experiments with 32P-end-labelled oligodeoxyribonucleotides, it is shown that treatment of DNA with the powerful oxidant dimethyldioxirane, followed by heating in piperidine, causes selective strand scission at the sites of guanine bases. The same specificity for cleavage at guanine was observed with a 45-mer labelled at either the 3′- or 5′-end and with a single and double stranded 34-mer. On account of its speed and operational simplicity, modification with dimethyldioxirane is proposed as a practicable alternative to conventional chemical sequencing procedures for locating guanine bases in DNA. © 1990.

引用

页码：87 / 94

页数：8

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