EVIDENCE THAT THE REPELLENT RECEPTOR FORM OF SENSORY RHODOPSIN-I IS AN ATTRACTANT SIGNALING STATE

被引：35

作者：

YAN, B

SPUDICH, JL

机构：

[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT ANAT & STRUCT BIOL,BRONX,NY 10461

[2] COLUMBIA UNIV,DEPT CHEM,NEW YORK,NY 10027

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1991年 / 54卷 / 06期

关键词：

D O I：

10.1111/j.1751-1097.1991.tb02125.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The lifetime of the Halobacterium halobium sensory rhodopsin I (SR-I photocycle intermediate S373 was modulated by incorporating retinal analogs into SR-I apoprotein in vitro and in vivo. Photocycles by SR-I analog pigments exhibit the same reaction scheme and similar formation rates, but different decay rates, of their S373-like species as monitored by flash spectroscopy in membrane vesicle suspensions. The attractant receptor signaling efficiencies determined by physiological measurements are proportional to the lifetimes of the S373-like intermediates, indicating that S373 is a physiological active conformation (signaling state) of the receptor. A model incorporating this finding into the SR-I photocycle is presented.

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页码：1023 / 1026

页数：4

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