GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (GAPDH, EC 1.2.1.12.) GENE-EXPRESSION IN 2 MALIGNANT HUMAN MAMMARY EPITHELIAL-CELL LINES - BT-20 AND MCF-7 - REGULATION OF GENE-EXPRESSION BY 1,25-DIHYDROXYVITAMIN-D3 (1,25-(OH)2D3)

被引：35

作者：

DESPREZ, PY

POUJOL, D

SAEZ, S

机构：

[1] Laboratoire de Biologie Médicale, Centre de Lutte contre le Cancer Léon Bérard, F-69373 Lyon Cedex 08

来源：

CANCER LETTERS | 1992年 / 64卷 / 03期

关键词：

GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; MALIGNANT HUMAN MAMMARY CELL LINES; 1,25-DIHYDROXYVITAMIN-D3;

D O I：

10.1016/0304-3835(92)90046-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key enzyme in the control of glycolysis. Its gene expression was analyzed in two breast cancer cell lines of human origin, BT-20 and MCF-7. We used a cDNA probe of 1.3 kb for Northern blot hybridization. It is found that GAPDH mRNA is overexpressed only in the poorly differentiated BT-20 cell line and that treatment of these cells by 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) stimulates GAPDH mRNA expression in a dose-and time-dependent manner. The present investigation on the BT-20 cells indicates that the expression of GAPDH is sensitive to 1,25-(OH)2D3 and up-regulated by low doses of this steroid.

引用

页码：219 / 224

页数：6

共 25 条

[1] INSULIN STIMULATES GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE GENE-EXPRESSION THROUGH CIS-ACTING DNA-SEQUENCES [J].