CORRELATION OF CYCLOSPORINE AND METABOLITE BINDING TO CYCLOPHILIN AND A 50 KDA BINDING-PROTEIN WITH INVITRO IMMUNOSUPPRESSION - A PRELIMINARY-REPORT

被引：10

作者：

DONNELLY, JG

RUSSELL, RL

PALASZYNSKI, EW

COPELAND, KR

YATSCOFF, RW

CHAN, MM

SOLDIN, SJ

机构：

[1] CHILDRENS HOSP,NATL MED CTR,DEPT LAB MED,111 MICHIGAN AVE NW,WASHINGTON,DC 20010

[2] GEORGE WASHINGTON UNIV,DEPT PHARMACOL,WASHINGTON,DC 20052

[3] GEORGE WASHINGTON UNIV,DEPT BIOCHEM,WASHINGTON,DC 20052

[4] GEORGE WASHINGTON UNIV,DEPT PEDIAT,WASHINGTON,DC 20052

[5] GEORGE WASHINGTON UNIV,DEPT PATHOL,WASHINGTON,DC 20052

[6] HLTH SCI CTR,DEPT CLIN CHEM,WINNIPEG R3A 1R9,MANITOBA,CANADA

[7] HLTH SCI CTR,DEPT MED,WINNIPEG R3A 1R9,MANITOBA,CANADA

来源：

CLINICAL BIOCHEMISTRY | 1991年 / 24卷 / 01期

关键词：

CYCLOSPORINE; METABOLITES; MECHANISM OF ACTION; IMMUNOSUPPRESSION; MIXED LYMPHOCYTE CULTURE; RECEPTORS;

D O I：

10.1016/0009-9120(91)90273-H

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Seven purified cyclosporine (CsA) metabolites were analyzed for binding to cyclophilin and to a 50 kDa protein purified from a JURKAT cell line. In addition, the potency of the seven metabolites, relative to CsA, was obtained using a primary mixed lymphocyte culture (MLC) suppression assay. CsA, M1, 17, and 21 were found to be immunosuppressive in the concentration range used (0-500 ng/mL). These results were then compared to protein binding. CsA and metabolite 17 (M17) bound to both proteins. Conversely, M1, 13, 21, and 26 bound only to cyclophilin, while M8 and M18 bound only to the 50 kDa protein.

引用

页码：71 / 74

页数：4

共 16 条

[1]

COPELAND KR, 1990, CLIN CHEM, V36, P225

[2] A RADIORECEPTOR ASSAY FOR THE MEASUREMENT OF CYCLOSPORINE ACTIVITY - A PRELIMINARY-REPORT [J].