MONOCLONAL-ANTIBODY TO A 35 KD EPIDERMAL PROTEIN INDUCES CELL DETACHMENT

被引：13

作者：

NEGI, M ^{[1
]}

LANE, AT ^{[1
]}

MCCOON, PE ^{[1
]}

FAIRLEY, JA ^{[1
]}

GOLDSMITH, LA ^{[1
]}

机构：

[1] UNIV ROCHESTER, SCH MED & DENT, DEPT MED, DERMATOL UNIT, ROCHESTER, NY 14642 USA

来源：

JOURNAL OF INVESTIGATIVE DERMATOLOGY | 1986年 / 86卷 / 06期

关键词：

D O I：

10.1111/1523-1747.ep12275639

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

A murin monoclonal antibody (ECS-1) was prepared from BALB/c mice immunized with trypsinized cultured human foreskin keratinocytes. The antibody showed a pattern suggestive of intercellular staining on the nucleated layers of normal human epidermis, adult palm, mouse lip epidermis, and cultured human keratinocytes. ECS-1 stained human fetal skin by 9 weeks estimated gestational age. ECS-1 reacted with a 35 kD protein extracted from neonatal foreskin epidermis and cultured human keratinocytes. The protein required Nonidet P-40 or sodium dodecyl sulfate and mercaptoethanol for solubilization. ECS-1 induced epidermal cell detachment which was enhanced by complement. ECS-1 shares characteristics with human pemphigus antibodies.

引用

页码：634 / 637

页数：4

共 19 条

[1]

GOLDSMITH LA, 1983, NORMAL ABNORMAL EPID, P3

[2] INVITRO COMPLEMENT ACTIVATION BY INTER-CELLULAR ANTIBODIES [J].

HASHIMOTO, T ;

SUGIURA, M ;

KURIHARA, S ;

NISHIKAWA, T .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1982, 78 (04) :316-318

[3]

JONES JCR, 1984, P NATL ACAD SCI-BIOL, V81, P2781, DOI 10.1073/pnas.81.9.2781

[4] COMPLEMENT-FIXATION BY PEMPHIGUS ANTIBODY .1. INVITRO FIXATION TO ORGAN AND TISSUE-CULTURE SKIN [J].

KAWANA, S ;

JANSON, M ;

JORDON, RE .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1984, 82 (05) :506-510

[5]

KAWANA S, 1985, CLIN EXP IMMUNOL, V61, P517

[6]

Labib R S, 1983, Clin Dermatol, V1, P82, DOI 10.1016/0738-081X(83)90024-X

[7] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[8] IDENTIFICATION OF BULLOUS PEMPHIGOID, PEMPHIGUS, LAMININ, AND ANCHORING FIBRIL ANTIGENS IN HUMAN-FETAL SKIN [J].

LANE, AT ;

HELM, KF ;

GOLDSMITH, LA .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1985, 84 (01) :27-30

[9] STRATIFICATION, SPECIALIZATION, AND PROLIFERATION OF PRIMARY KERATINOCYTE CULTURES - EVIDENCE OF A FUNCTIONING INVITRO EPIDERMAL-CELL SYSTEM [J].

MARCELO, CL ;

KIM, YG ;

KAINE, JL ;

VOORHEES, JJ .

JOURNAL OF CELL BIOLOGY, 1978, 79 (02) :356-370

[10] ONTOGENY OF PEMPHIGUS AND BULLOUS PEMPHIGOID ANTIGENS IN HUMAN SKIN [J].

MULLER, HK ;

KALNINS, R ;

SUTHERLAND, RC .

BRITISH JOURNAL OF DERMATOLOGY, 1973, 88 (05) :443-446

← 1 2 →