DETERMINATION OF SUPEROXIDE DISMUTASE-LIKE ACTIVITY OF COPPER(II) COMPLEXES - RELEVANCE OF THE SPECIATION FOR THE CORRECT INTERPRETATION OF INVITRO O2- SCAVENGER ACTIVITY

被引：60

作者：

COSTANZO, LL

DEGUIDI, G

GIUFFRIDA, S

RIZZARELLI, E

VECCHIO, G

机构：

[1] UNIV CATANIA, DIPARTIMENTO SCI CHIM, VIALE A DORIA 8, I-95125 CATANIA, ITALY

[2] CNR, IST STUDIO SOSTANZE NAT INTERESSE ALIMENTARE & CHI, CATANIA, ITALY

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 1993年 / 50卷 / 04期

关键词：

D O I：

10.1016/0162-0134(93)80054-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The superoxide dismutase activity of several copper(II) complexes of linear and cyclic dipeptides has been measured. The results of a classic indirect method (xanthine-xanthine oxidase) have been compared with those obtained by generation of the superoxide radical through 2-(-3-benzoylphenyl)propionic acid (ketoprofen) photolysis. A simulation approach, based on the knowledge of the stability constants of the different complex species existing in experimental conditions. has allowed us to obtain the correct speciation and to use these data to calculate the pertinent catalytic constants.

引用

页码：273 / 281

页数：9

共 53 条

[1]

ALLEN AO, 1982, SUPEROXIDE DISMUTASE, V1, P125

[2] CATALYTIC ACTIVITY STUDIES OF SOME COPPER(II)-HISTIDINE-CONTAINING DIPEPTIDE COMPLEXES ON AQUEOUS SUPEROXIDE ION DISMUTATION [J].