ANALYSIS OF POPPY STRAW AND POPPY STRAW CONCENTRATE BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：9

作者：

GOMEZSERRANILLOS, P ^{[1
]}

CARRETERO, E ^{[1
]}

VILLAR, A ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID,FAC FARM,DEPT FARMACOL,E-28040 MADRID,SPAIN

来源：

PHYTOCHEMICAL ANALYSIS | 1994年 / 5卷 / 01期

关键词：

POPPY STRAW; ALKALOID DETERMINATION; REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

D O I：

10.1002/pca.2800050105

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method of reversed-phase high-performance liquid chromatography using an elution gradient has been developed to analyse morphine, codeine, thebaine, and oripavine - this last-mentioned substance for the first time - in samples of poppy straw and poppy straw concentrate. Gradient elution of the octadecylsilane column was performed: the gradient was from 10 to 30% of solvent A (methanol) in solvent B (containing 67% of 0.5% aqueous ammonium acetate solution containing diethylamine, and 33% of methanol) over a 25 min period. The four alkaloids were identified and quantified with the aid of an internal standard.

引用

页码：15 / 18

页数：4

共 35 条

[1]

AKHILA A, 1983, J CHROMATOGR, V253, P236

[2] SEPARATION OF 8 ALKALOIDS AND MECONIC ACID AND QUANTITATION OF 5 PRINCIPAL ALKALOIDS IN GUM OPIUM BY GRADIENT REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

AYYANGAR, NR ;

BHIDE, SR .

JOURNAL OF CHROMATOGRAPHY, 1988, 436 (03) :455-465

[3]

AYYANGAR NR, 1986, J CHROMATOGR, V336, P435

[4] THE SEPARATION AND QUANTITATION OF THE NARCOTIC COMPONENTS OF ILLICIT HEROIN USING REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

BAKER, PB ;

GOUGH, TA .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1981, 19 (10) :483-489

[5]

BEASLEY TH, 1974, J ASSOC OFF ANA CHEM, V57, P85

[6]

BILLIET HAH, 1986, J CHROMATOGR, V386, P351

[7] QUANTITATIVE-DETERMINATION OF MORPHINE IN PAREGORIC USP BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].

DASGUPTA, V .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1976, 65 (11) :1697-1698

[8]

DOGAN M, 1986, Fitoterapia, V57, P257

[9] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF ALKALOIDS FROM PAPAVER-SOMNIFERUM ON A ZORBAX NH2 ANALYTICAL COLUMN [J].

DONER, LW ;

HSU, AF .

JOURNAL OF CHROMATOGRAPHY, 1982, 253 (01) :120-123

[10]

EVREN N, 1985, GAZI U ECZACILIK FAK, V2, P67

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