CAPILLARY ZONE ELECTROPHORESIS OF ALDOSE ENANTIOMERS - SEPARATION AFTER DERIVATIZATION WITH S-(-)-1-PHENYLETHYLAMINE

被引：60

作者：

NOE, CR

FREISSMUTH, J

机构：

[1] Institute of Pharmaceutical Chemistry, Christian Doppler Laboratory for Chemistry of Chiral Compounds, Johann Wolfgang Goethe University, D-60439 Frankfurt

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 704卷 / 02期

关键词：

D O I：

10.1016/0021-9673(95)00237-H

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the analysis of monosaccharide enantiomers of the aldose-type is described. Aminoalditols resulting from derivatization with S-(-)-1-phenylethylamine were subjected to capillary electrophoresis in a berate buffer. Standard on-column UV detection at 200 nm was applied. Studies of the dependence of separation selectivity and electrophoretic mobility on temperature, electrolyte composition and pH were carried out. Single-run resolution of all 16 derivatized aldohexoses and 3 out of 4 aldotetroses was achieved at pH 10.3 in a 50 mM berate buffer using acetonitrile as organic modifier.

引用

页码：503 / 512

页数：10

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