CAPILLARY ZONE ELECTROPHORESIS OF ALDOSE ENANTIOMERS - SEPARATION AFTER DERIVATIZATION WITH S-(-)-1-PHENYLETHYLAMINE

被引:60
作者
NOE, CR
FREISSMUTH, J
机构
[1] Institute of Pharmaceutical Chemistry, Christian Doppler Laboratory for Chemistry of Chiral Compounds, Johann Wolfgang Goethe University, D-60439 Frankfurt
关键词
D O I
10.1016/0021-9673(95)00237-H
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A method for the analysis of monosaccharide enantiomers of the aldose-type is described. Aminoalditols resulting from derivatization with S-(-)-1-phenylethylamine were subjected to capillary electrophoresis in a berate buffer. Standard on-column UV detection at 200 nm was applied. Studies of the dependence of separation selectivity and electrophoretic mobility on temperature, electrolyte composition and pH were carried out. Single-run resolution of all 16 derivatized aldohexoses and 3 out of 4 aldotetroses was achieved at pH 10.3 in a 50 mM berate buffer using acetonitrile as organic modifier.
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页码:503 / 512
页数:10
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