A METHOD FOR ANALYZING SISTER-CHROMATID EXCHANGE IN MOUSE PREIMPLANTATION EMBRYOS

被引：7

作者：

ELBLING, L ^{[1
]}

COLOT, M ^{[1
]}

机构：

[1] VIENNA UNIV, INST APPL & EXPTL ONCOL, BORSCHKEGASSE 8A, A-1090 VIENNA, AUSTRIA

来源：

MUTATION RESEARCH | 1985年 / 147卷 / 1-2期

关键词：

D O I：

10.1016/0165-1161(85)90014-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Analysis of sister-chromatid exchange (SCE) was shown to be a sensitive and reproducible method for detecting the action of mutagens and carcinogens. A reliable technique was established which allows to perform SCE in preimplantation embryos in order to make the pre-uterine stages of development accessible to routine detection of DNA damage. Using the mouse strain and technique described, .apprx. 30-40% of mice will mate successfully after synchronization and spontaneous ovulation. From 3 pregnant females, about 30 4-8-cell embryos will be obtained, representing one experimental group providing .apprx. 50-80 2-S-phase labeled metaphases with a SCE frequency baseline below 6 exchanges.

引用

页码：23 / 28

页数：6

共 32 条

[1] ANALYSIS OF SISTER CHROMATID EXCHANGE FORMATION INVIVO IN MOUSE SPERMATOGONIA AS A NEW TEST SYSTEM FOR ENVIRONMENTAL MUTAGENS [J].

ALLEN, JW ;

LATT, SA .

NATURE, 1976, 260 (5550) :449-451

[2] SIMPLIFIED TECHNIQUE FOR INVIVO ANALYSIS OF SISTER-CHROMATID EXCHANGES USING 5-BROMODEOXYURIDINE TABLETS [J].

ALLEN, JW ;

SHULER, CF ;

MENDES, RW ;

LATT, SA .

CYTOGENETICS AND CELL GENETICS, 1977, 18 (04) :231-237

[3] SISTER-CHROMATID EXCHANGE ANALYSES IN RODENT MATERNAL, EMBRYONIC AND EXTRA-EMBRYONIC TISSUES - TRANS-PLACENTAL AND DIRECT MUTAGEN EXPOSURES [J].

ALLEN, JW ;

ELNAHASS, E ;

SANYAL, MK ;

DUNN, RL ;

GLADEN, B ;

DIXON, RL .

MUTATION RESEARCH, 1981, 80 (02) :297-311

[4] EARLY MOUSE EMBRYOS EXHIBIT STRAIN VARIATION IN RADIATION-INDUCED SISTER-CHROMATID EXCHANGE - RELATIONSHIP WITH DNA-REPAIR [J].

BENNETT, J ;

PEDERSEN, RA .

MUTATION RESEARCH, 1984, 126 (02) :153-157

[5]

Biggers J. D., 1971, Methods in mammalian embryology, P86

[6]

GARNER W, 1974, J EMBRYOL EXP MORPH, V32, P849

[7] EFFECT OF 5-BROMODEOXYURIDINE ON PRE-IMPLANTATION MOUSE EMBRYO DEVELOPMENT [J].

GOLBUS, MS ;

EPSTEIN, CJ .

DIFFERENTIATION, 1974, 2 (03) :143-149

[8] SISTER-CHROMATID EXCHANGE AND MICRONUCLEUS INDUCTION AS INDICATORS OF GENETIC-DAMAGE IN MATERNAL AND FETAL CELLS [J].

HENDERSON, L ;

COLE, R ;

COLE, J ;

COLE, H ;

AGHAMOHAMMADI, Z ;

REGAN, T .

MUTATION RESEARCH, 1984, 126 (01) :47-52

[9] SISTER CHROMATID EXCHANGES IN BONE-MARROW CELLS OF MICE MAINTAINED ON TRITIATED-WATER [J].

IKUSHIMA, T ;

BENZ, RD ;

CARSTEN, AL .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1984, 45 (03) :251-256

[10] INDUCTION OF SISTER CHROMATID EXCHANGES BY CHEMICAL MUTAGENS AND ITS POSSIBLE RELEVANCE TO DNA-REPAIR [J].

KATO, H .

EXPERIMENTAL CELL RESEARCH, 1974, 85 (02) :239-247

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