LOCALIZATION OF INSULIN-RECEPTOR MESSENGER-RNA IN RAT-BRAIN BY INSITU HYBRIDIZATION

被引:340
作者
MARKS, JL
PORTE, D
STAHL, WL
BASKIN, DG
机构
[1] UNIV WASHINGTON,DEPT BIOL STRUCT,SEATTLE,WA 98195
[2] UNIV WASHINGTON,DEPT PHYSIOL & BIOPHYS,SEATTLE,WA 98195
[3] UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98195
关键词
D O I
10.1210/endo-127-6-3234
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Insulin receptor mRNA was demonstrated in rat brain slices by in situ hybridization with three35S-oligonucleotide probes and contact film autoradiography. Specificity was confirmed by showing that (a) excess unlabeled probe abolished the signal, (b) an oligonucleotide probe for rat neuropeptide Y mRNA showed a different distribution of hybridization signal, and (c) the distribution of insulin receptor binding was consistent with the distribution of insulin receptor mRNA. Insulin receptor mRNA was most abundant in the granule cell layers of the olfactory bulb, cerebellum and dentate gyrus, in the pyramidal cell body layers of the pyriform cortex and hippocampus, in the choroid plexus and in the arcuate nucleus of the hypothalamus. © 1990 by The Endocrine Society.
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页码:3234 / 3236
页数:3
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