POLYMERASE CHAIN-REACTION ASSAY FOR DIAGNOSIS OF POTENTIALLY TOXINOGENIC CORYNEBACTERIUM-DIPHTHERIAE STRAINS - CORRELATION WITH ADP-RIBOSYLATION ACTIVITY ASSAY

被引：51

作者：

HAUSER, D ^{[1
]}

POPOFF, MR ^{[1
]}

KIREDJIAN, M ^{[1
]}

BOQUET, P ^{[1
]}

BIMET, F ^{[1
]}

机构：

[1] INST PASTEUR,IDENTIFICAT BACTERIENNES LAB,F-75724 PARIS 15,FRANCE

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 10期

关键词：

D O I：

10.1128/JCM.31.10.2720-2723.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have developed a polymerase chain reaction assay for the clinical diagnosis of potentially toxinogenic strains of Corynebacterium diphtheriae, the causative agent of diphtheria. A 910-bp amplification product, overlapping a DNA portion encoding both fragments of the diphtheria toxin, has been found in 28 among the 36 strains tested. In addition, effective toxin production, as evidenced by the ability of bacterial culture supernatants to ADP ribosylate eukaryotic elongation factor 2, was determined. In every case, the presence of an amplification product correlated with an ADP-ribosylation activity, thus confirming the diagnosis. The polymerase chain reaction assay herein described is very rapid (2 h) compared with the Elek immunodiffusion test or the guinea pig lethality test. It can provide a convenient and reliable method for laboratories involved in the identification of toxinogenic corynebacteria.

引用

页码：2720 / 2723

页数：4

共 13 条

[1] DNA RELATIONSHIPS AMONG SOME TOX-BEARING CORYNE BACTERIOPHAGES [J].

BUCK, GA ;

CROSS, RE ;

WONG, TP ;

LOERA, J ;

GROMAN, N .

INFECTION AND IMMUNITY, 1985, 49 (03) :679-684

[2]

DRAZIN R, 1971, J BIOL CHEM, V246, P1504

[3] THE RECOGNITION OF TOXICOGENIC BACTERIAL STRAINS INVITRO [J].

ELEK, SD .

BRITISH MEDICAL JOURNAL, 1948, 1 (4549) :493-496

[4] STUDIES ON THE VIRULENCE OF BACTERIOPHAGE-INFECTED STRAINS OF CORYNEBACTERIUM-DIPHTHERIAE [J].

FREEMAN, VJ .

JOURNAL OF BACTERIOLOGY, 1951, 61 (06) :675-688

[5] EXPRESSION OF DIPHTHERIA TOXIN GENES CARRIED BY INTEGRATED AND NONINTEGRATED PHAGE BETA [J].

GILL, DM ;

UCHIDA, T ;

SINGER, RA .

VIROLOGY, 1972, 50 (03) :664-668

[6]

GILL DM, 1971, J BIOL CHEM, V246, P1492

[7] DETECTION AND EXPRESSION OF DNA HOMOLOGOUS TO THE TOX GENE IN NONTOXINOGENIC ISOLATES OF CORYNEBACTERIUM-DIPHTHERIAE [J].

GROMAN, N ;

CIANCIOTTO, N ;

BJORN, M ;

RABIN, M .

INFECTION AND IMMUNITY, 1983, 42 (01) :48-56

[8] CORYNEBACTERIUM-ULCERANS AND CORYNEBACTERIUM-PSEUDOTUBERCULOSIS RESPONSES TO DNA PROBES DERIVED FROM CORYNEPHAGE-BETA AND CORYNEBACTERIUM-DIPHTHERIAE [J].

GROMAN, N ;

SCHILLER, J ;

RUSSELL, J .

INFECTION AND IMMUNITY, 1984, 45 (02) :511-517

[9]

MURPHY JR, 1985, CURR TOP MICROBIOL, V118, P235

[10] RAPID SCREENING FOR TOXIGENIC CORYNEBACTERIUM-DIPHTHERIAE BY THE POLYMERASE CHAIN-REACTION [J].

PALLEN, MJ .

JOURNAL OF CLINICAL PATHOLOGY, 1991, 44 (12) :1025-1026

← 1 2 →