MODELING OF ENZYMATIC-REACTION WITH EMULSION LIQUID MEMBRANE

A mathematical model is developed for analyzing the rate of reaction using an enzyme encapsulated by water/oil emulsion. The proposed mechanism of enzyme emulsion-liquid-membrane model is based on the simple-diffusion (type 1) facilitation of the permeation-consumption of substrate and the carrier-mediated (type 2) facilitation of the generation-permeation of product. Input to the model is the phase and chemical equilibria at the interface of external-emulsion and internal-membrane phase. The developed model takes into account the reactivity of internal enzyme, intra-emulsion diffusion and peri-emulsion mass transfer for both substrate and product. All parameters needed for modeling can be estimated without the use of adjustable parameters, but only the thermodynamic, transport. emulsion and enzyme properties. The proposed model predicts satisfactorily the experimental results of an enzymatic reaction in a batch system under various conditions.