SYNTHESIS OF L-PROLINE FROM THE RACEMATE BY COUPLING OF ENZYMATIC ENANTIOSPECIFIC OXIDATION AND CHEMICAL NONENANTIOSPECIFIC REDUCTION

被引：35

作者：

HUH, JW ^{[1
]}

YOKOIGAWA, K ^{[1
]}

ESAKI, N ^{[1
]}

SODA, K ^{[1
]}

机构：

[1] KYOTO UNIV,INST CHEM RES,UJI,KYOTO 611,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1992年 / 74卷 / 03期

关键词：

D O I：

10.1016/0922-338X(92)90082-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed a new procedure for the synthesis of L-proline from the racemate by means of a coupled system of D-amino acid oxidase and sodium borohydride reactions. In this system, D-proline in the racemate is enantiospecifically oxidized to form DELTA1-pyrroline-2-carboxylic acid, which is immediately reduced to DL-proline. D-Proline is eventually exhausted and L-proline is exclusively synthesized in the reaction mixture with a yield of more than 98%. The proline isolated from the reaction mixture was 100% optically pure.

引用

页码：189 / 190

页数：2

共 8 条

[1]

Chibata I, 1976, Methods Enzymol, V44, P746

[2] ENZYMATIC CONVERSION OF DL-ALPHA-AMINO-EPSILON-CAPROLACTUM INTO L-LYSINE .6. CONVERSION OF D-ALPHA-AMINO-EPSILON-CAPROLACTUM INTO L-LYSINE USING BOTH YEAST-CELLS AND BACTERIAL-CELLS [J].