CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF PORCINE RIBONUCLEASE INHIBITOR, A PROTEIN WITH LEUCINE-RICH REPEATS

被引：15

作者：

KOBE, B

DEISENHOFER, J

机构：

[1] Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75235-9050

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 231卷 / 01期

关键词：

RIBONUCLEASE INHIBITOR; LEUCINE-RICH REPEATS; CRYSTALLIZATION; PRELIMINARY X-RAY DATA;

D O I：

10.1006/jmbi.1993.1263

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ribonuclease inhibitor was purified from pig liver and crystallized at 21°C from solutions containing dithiothreitol as an additive and ammonium sulfate, lithium sulfate or combinations of both as precipitants. Crystals have the symmetry of the tetragonal space group I4 with a = 134.9 Å and c = 83.6 Å, and diffract to better than 3 Å resolution. Self rotation functions and packing density of the crystals are consistent with two molecules in the asymmetric unit. © 1993 Academic Press, Inc.

引用

页码：137 / 140

页数：4

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