EVALUATION OF HORSERADISH-PEROXIDASE AS AN INTRACELLULAR STAIN IN ELECTROPHYSIOLOGICAL RECORDING

被引：4

作者：

BALL, A

AMMERMULLER, J

WEILER, R

机构：

[1] UNIV MUNICH,INST ZOOL,W-8000 MUNICH 2,GERMANY

[2] UNIV OLDENBURG,DEPT NEUROBIOL,W-2900 OLDENBURG,GERMANY

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1990年 / 31卷 / 01期

基金：

英国医学研究理事会;

关键词：

Electrophysiology; Fixation; Horseradish peroxidase; Intracellular; Iontophoresis; Staining;

D O I：

10.1016/0165-0270(90)90006-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Iontophoresis of horseradish peroxidase into cells during intracellular electrophysiological recording to visualize the neurons recorded from is a powerful technique, but its success depends on a number of critical factors. The described agar injection technique is a simple method for the evaluation of several parameters at once, using procedures which closely resemble recording from, fixing, and processing to visualize the HRP in experimental tissue. © 1990.

引用

页码：35 / 41

页数：7

共 19 条

[1]

ALHEID GF, 1981, NEUROANATOMICAL TRAC, P91

[2]

DELCOMYN F, 1983, SPRINGER SERIES EXPT, pCH4

[3]

DJAMGOZ MBA, 1987, EXP BIOL, V46, P203

[4] NEURONAL ARCHITECTURE OF ON AND OFF PATHWAYS TO GANGLION-CELLS IN CARP RETINA [J].