MUTATIONAL MAPPING OF RAS-RESPONSIVE DOMAINS OF THE SACCHAROMYCES-CEREVISIAE ADENYLYL CYCLASE

被引：52

作者：

COLICELLI, J ^{[1
]}

FIELD, J ^{[1
]}

BALLESTER, R ^{[1
]}

CHESTER, N ^{[1
]}

YOUNG, D ^{[1
]}

WIGLER, M ^{[1
]}

机构：

[1] COLD SPRING HARBOR LAB,POB 100,COLD SPRING HARBOR,NY 11724

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 06期

关键词：

D O I：

10.1128/MCB.10.6.2539

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Large deletion and small insertion mutations in the adenylyl cyclase gene of Saccharomyces cerevisiae were used to map regions required for activation by RAS protein in vitro. The amino-terminal 605 amino acids were found to be dispensable for responsiveness to RAS protein. All other deletions in adenylyl cyclase destroyed its ability to respond to RAS. Small insertion mutations within the leucine-rich repeat region also prevented RAS responsiveness, while other insertions did not.

引用

页码：2539 / 2543

页数：5

共 25 条

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