CELL-SURFACE CHARACTERISTICS OF LACTOCOCCUS-LACTIS HARBORING PCI528, A 46 KB PLASMID ENCODING INHIBITION OF BACTERIOPHAGE ADSORPTION

被引：33

作者：

LUCEY, M

DALY, C

FITZGERALD, GF

机构：

[1] NATL UNIV IRELAND UNIV COLL CORK,DEPT FOOD MICROBIOL,CORK,IRELAND

[2] NATL UNIV IRELAND UNIV COLL CORK,NATL FOOD BIOTECHNOL CTR,CORK,IRELAND

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1992年 / 138卷

关键词：

D O I：

10.1099/00221287-138-10-2137

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

pCI528 is a 46 kb plasmid, originally isolated from Lactococcus lactis subsp. cremoris UC503, which causes a decrease in bacteriophage adsorption in lactococcal strains. Cell surface characteristics of two pCI528-containing strains, L. lactis subsp. cremoris UC503 and L. lactis subsp. lactis UC505, were compared with those of their isogenic pCI528-cured derivatives, UC563 and MG1363, respectively. Following centrifugation, strains containing pCI528 produced a loose fluffy pellet. The presence of pCI528 also caused a 'hard' colony morphology on agar media. The fluffy pellet effect mediated by pCI528 could be eliminated by washing cells in 0.05 M-NaOH, which also restored full sensitivity to phage in the L. lactis subsp. lactis UC505 background. GLC analysis of cell wall material indicated that pCI528-containing hosts had significantly elevated levels of both galactose and rhamnose. Marked alterations in the cell surface of strains harbouring pCI528 were observed in electron micrographs.

引用

页码：2137 / 2143

页数：7

共 27 条

[1] 2 PLASMID-DETERMINED RESTRICTION AND MODIFICATION SYSTEMS IN STREPTOCOCCUS-LACTIS
CHOPIN, A
CHOPIN, MC
MOILLOBATT, A
LANGELLA, P
[J]. PLASMID, 1984, 11 (03) : 260 - 263
[2] COFFEY A, 1991, GENETICS AND MOLECULAR BIOLOGY OF STREPTOCOCCI, LACTOCOCCI, AND ENTEROCOCCI, P131
[3] COFFEY AG, 1989, NETH MILK DAIRY J, V43, P229
[4] COGAN MTM, 1991, B INT DAIRY FEDERATI, V263, P16
[5] COSTELLO VA, 1988, THESIS NATIONAL U IR
[6] DALY C, 1987, STREPTOCOCCAL GENETI, P259
[7] DEVOS WM, 1984, FEMS MICROBIOL LETT, V23, P175, DOI 10.1111/j.1574-6968.1984.tb01057.x
[8] EVERSON TC, 1991, B INT DAIRY FED, V263, P24
[9] GASSON MJ, 1983, J BACTERIOL, V154, P1
[10] THE CONJUGATIVE PLASMID PTR2030 ENCODES 2 BACTERIOPHAGE DEFENSE-MECHANISMS IN LACTOCOCCI, RESTRICTION MODIFICATION (R+/M+) AND ABORTIVE INFECTION (HSP+)
HILL, C
PIERCE, K
KLAENHAMMER, TR
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (09) : 2416 - 2419

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