DIVERSITY AND RELATIVE STRENGTH OF TANDEM PROMOTERS FOR THE ANTIBIOTIC-RESISTANCE GENES OF SEVERAL INTEGRONS

被引：199

作者：

LEVESQUE, C

BRASSARD, S

LAPOINTE, J

ROY, PH

机构：

[1] CHUL, CTR RECH, SERV INFECT LAB, Ste Foy G1V 4G2, PQ, CANADA

[2] UNIV LAVAL, FAC SCI & GENIE, DEPT BIOCHIM, Ste Foy G1K 7P4, PQ, CANADA

来源：

GENE | 1994年 / 142卷 / 01期

基金：

英国医学研究理事会;

关键词：

GENE CLONING; SITE-SPECIFIC RECOMBINATION; TRANSPOSON; NATURAL EXPRESSION VECTORS; CAT ASSAY; GENE EXPRESSION; PROMOTER PROBE VECTOR;

D O I：

10.1016/0378-1119(94)90353-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The integron is a new type of mobile element containing one or more antibiotic-resistance-encoding genes site-specifically integrated as cassettes. The integrated genes are expressed from a common promoter region located in an adjacent conserved segment. Sequence analysis has revealed the existence of four versions of the integron promoters. In this study, we have determined the relative strength of the different integron promoters and compared their activity with that of the tac promoter. Each version of the promoter was cloned upstream from a promoter-less chloramphenicol acetyltransferase-encoding gene (cat) in plasmid pKK232-8. CAT activity was used to measure transcriptional expression from the promoters of the antibiotic-resistance operon. The strongest promoter is the Version (TTGACAN(17)TAAACT) found in plasmid R388 and in transposon Tn1696. This promoter is six times more efficient than the derepressed tac promoter.

引用

页码：49 / 54

页数：6

共 20 条

[1] CHARACTERIZATION OF IN0 OF PSEUDOMONAS-AERUGINOSA PLASMID PVS1, AN ANCESTOR OF INTEGRONS OF MULTIRESISTANCE PLASMIDS AND TRANSPOSONS OF GRAM-NEGATIVE BACTERIA [J].