INHIBITION OF THE DIFFERENTIATION OF HUMAN MYELOID CELL-LINES BY REDOX CHANGES INDUCED THROUGH GLUTATHIONE DEPLETION

被引:47
作者
ESPOSITO, F
AGOSTI, V
MORRONE, G
MORRA, F
CUOMO, C
RUSSO, T
VENUTA, S
CIMINO, F
机构
[1] UNIV NAPLES FEDERICO II, DIPARTIMENTO BIOCHIM & BIOTECNOL MED, I-80131 NAPLES, ITALY
[2] UNIV REGGIO CALABRIA, DIPARTIMENTO MED SPERIMENTALE & CLIN, I-88100 CATANZARO, ITALY
关键词
D O I
10.1042/bj3010649
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have investigated the effect of redox changes in vivo on the differentiation of two human myeloid cell lines, HL-60 and KG-1. The glutathione-depleting agent diethyl maleate (DEM) prevented the development of differentiated features in response to phorbol esters, including adherence of the cells to plastic surfaces and repression of the myeloperoxidase and CD34 genes. Moreover, DEM abolished phorbol 12-myristate 13-acetate-induced activation of the transcription factors AP-1 and Egr-1, suggesting that inhibition of differentiation may be due, at least in part, to redox modifications of these proteins.
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页码:649 / 653
页数:5
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