CELLULAR COMPOSITION OF MILKY SPOTS IN THE HUMAN GREATER OMENTUM - AN IMMUNOCHEMICAL AND ULTRASTRUCTURAL-STUDY

被引:95
作者
KRIST, LFG
EESTERMANS, IL
STEENBERGEN, JJE
HOEFSMIT, ECM
CUESTA, MA
MEYER, S
BEELEN, RHJ
机构
[1] FREE UNIV AMSTERDAM HOSP,DEPT SURG,1007 MB AMSTERDAM,NETHERLANDS
[2] FREE UNIV AMSTERDAM HOSP,FAC MED,DEPT CELL BIOL,1007 MB AMSTERDAM,NETHERLANDS
来源
ANATOMICAL RECORD | 1995年 / 241卷 / 02期
关键词
HUMAN GREATER OMENTUM; MILKY SPOTS; MACROPHAGES; LYMPHOCYTES; LIGHT MICROSCOPY; ELECTRON MICROSCOPY;
D O I
10.1002/ar.1092410204
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Background: Milky spots in the greater omentum of some animals are well organized perivascular infiltrates of leucocytes, and are considered to have characteristics of secondary lymphoid tissue. To determine whether milky spots in the human greater omentum can also be regarded as secondary lymphoid tissue, we studied milky spots in an unstimulated state. Methods: Patients were selected on the basis of absence of disease in the peritoneal cavity that might influence the state of the milky spots. Using monoclonal antibodies against macrophages, B-lymphocytes and T-lymphocytes, and immunoperoxidase labeling, the number of these cells and their location in milky spots were studied by light microscopy. However, the stromal components of the greater omentum, especially those within the milky spots, were studied by electron microscopy. Results: Milky spots in the human greater omentum are relatively uniform vascularized accumulations of mononuclear cells comprising macrophages (67.9% +/- 9.4, mean a standard deviation), B-cells (10.1% +/- 3.4), T-cells (10.2% +/- 3.7), and mast cells. However, no special B-cell and T-cell areas could be distinguished. On the ultrastructural level it was demonstrated that macrophages are present in different stages of maturation and can enter or leave the milky spots. Furthermore, no cells characteristic of secondary lymphoid organs, such as interdigitating cells or follicular dendritic cells, were seen. Conclusions: These data indicate that unstimulated milky spots in the human greater omentum are to a great extent just a preformed specific accumulation of primarily macrophages within the stroma of the greater omentum, and therefore, cannot be regarded as true secondary lymphoid tissue. Milky spots could serve as a gateway for, as well as a provider of peritoneal macrophages when the intra-abdominal status so requires. Finally, the data from this study are compared with the data of other studies of human milky spots and those in animals. (C) 1995 Wiley-Liss, Inc.
引用
收藏
页码:163 / 174
页数:12
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