CYTOSYLGLUCURONIC ACID SYNTHASE (CYTOSINE-UDP-GLUCURONOSYLTRANSFERASE) FROM STREPTOMYCES GRISEOCHROMOGENES, THE FIRST PROKARYOTIC UDP-GLUCURONOSYLTRANSFERASE

被引：17

作者：

GOULD, SJ ^{[1
]}

GUO, JC ^{[1
]}

机构：

[1] OREGON STATE UNIV,DEPT BIOCHEM & BIOPHYS,CORVALLIS,OR 97331

来源：

JOURNAL OF BACTERIOLOGY | 1994年 / 176卷 / 05期

关键词：

D O I：

10.1128/jb.176.5.1282-1286.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Cytosylglucuronic acid synthase (cytosine:UDP-glucuronosyltransferase), the first prokaryotic UDP-GT and a key enzyme in the biosynthesis of the antibiotic blasticidin S, was purified 870-fold. It has optimum activity at a pH of 8.4 to 8.6, K(m)s of 6.0 (UDP-glucuronic acid) and 243 (cytosine) mu M, and a maximum rate of metabolism of 14.6 mu mol/min/mg. The apparent M(r) is 43,000. Activity was slightly enhanced by Mg2+ or Ca2+ but was not inhibited by EDTA. Activity was strongly inhibited by UDP. Cytosylglucuronic acid differs from eukaryotic UDP-glucuronosyltransferases in being a soluble protein with no apparent phospholipid requirement.

引用

页码：1282 / 1286

页数：5

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