GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC DETERMINATION OF 5-HYDROXYMETHYLURACIL IN HUMAN URINE BY STABLE-ISOTOPE DILUTION

被引：19

作者：

FAURE, H ^{[1
]}

INCARDONA, MF ^{[1
]}

BOUJET, C ^{[1
]}

CADET, J ^{[1
]}

DUCROS, V ^{[1
]}

FAVIER, A ^{[1
]}

机构：

[1] SESAM,DEPT RECH FONDAMENTALE MAT CONDENSEE,LES ACIDES NUCL LAB,F-38041 GRENOBLE,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 616卷 / 01期

关键词：

D O I：

10.1016/0378-4347(93)80465-G

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the determination of 5-hydroxymethyluracil in urine is described. 5-Hydroxymethyluracil was extracted by reversed-phase chromatography and quantified by gas chromatography-mass spectrometry as tert.-butyldimethylsilyl derivative. Since natural 5-hydroxymethyluracil contained ca. 22% of M + 2 species, an internal standard consisting of [1,3-N-15(2),5-H-2(2)]hydroxymethyluracil was used to correct losses during extraction, evaporation and derivatization. Between-run precision of this method was 7.79%. and concentrations as low as 1.87 nM could be measured. This sensitivity and precision could not be obtained with trimethylsilyl derivatives.

引用

页码：1 / 7

页数：7

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