THE SINGLE-CELL GEL-ELECTROPHORESIS ASSAY (COMET ASSAY) - A EUROPEAN REVIEW

被引：758

作者：

MCKELVEYMARTIN, VJ

GREEN, MHL

SCHMEZER, P

POOLZOBEL, BL

DEMEO, MP

COLLINS, A

机构：

[1] ROWETT RES INST,ABERDEEN AB2 9SB,SCOTLAND

[2] UNIV SUSSEX,MRC,CELL MUTAT UNIT,BRIGHTON BN1 9RR,ENGLAND

[3] GERMAN CANC RES CTR,DEPT CARCINOGENESIS & CHEMOTHERAPY,W-6900 HEIDELBERG,GERMANY

[4] FED RES CTR NUTR,INST HYG & TOXICOL,W-7500 KARLSRUHE,GERMANY

[5] FAC PHARM MARSEILLE,MICROBIOL LAB,F-13385 MARSEILLE 5,FRANCE

来源：

MUTATION RESEARCH | 1993年 / 288卷 / 01期

关键词：

SINGLE CELL GEL ELECTROPHORESIS ASSAY; COMET ASSAY; SCGE; DNA BREAKAGE; MEASUREMENT;

D O I：

10.1016/0027-5107(93)90207-V

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The single cell gel electrophoresis (SCGE) assay is a rapid, simple, visual and sensitive technique for measuring DNA breakage in individual mammalian cells. Here we review the development of the SCGE assay (with particular reference to the alkaline version), existing protocols for the detection and analysis of comets, the relevant underlying principles determining the behaviour of DNA, and the potential applications of the technique.

引用

页码：47 / 63

页数：17

共 40 条

[1] RADIATION-INDUCED SINGLE-STRAND BREAKS IN DNA DETERMINED BY RATE OF ALKALINE STRAND SEPARATION AND HYDROXYLAPATITE CHROMATOGRAPHY - ALTERNATIVE TO VELOCITY SEDIMENTATION [J].