A POLYPURINE SEQUENCE THAT ACTS AS A 5' MESSENGER-RNA STABILIZER IN BACILLUS-SUBTILIS

A segment of early RNA from Bacillus subtilis bacteriophage SP82 was shown to function as a 5' stabilizer in B, subtilis, Several heterologous RNA sequences were stabilized by the presence of the SP82 sequence at the 5' end, and expression of downstream coding sequences was increased severalfold, The SP82 RNA segment encodes a B, subtilis RNase III cleavage site, but cleavage by B, subtilis RNase III was not required for stabilization, The sequence that specifies 5' stabilizer function was localized to a polypurine sequence that resembles a ribosome binding site, The ability of the SP82 sequence to stabilize downstream RNA was dependent on its position relative to the 5' end of the RNA. These results demonstrate the existence of a new type of 5' stabilizer in B. subtilis and indicate that attack at the 5' end is a principal mechanism for initiation of mRNA decay in B, subtilis.