THE EFFECT OF DIETARY POLYENYLPHOSPHATIDYLCHOLINE ON MICROSOMAL DELTA-6-DESATURASE ACTIVITY, FATTY-ACID COMPOSITION, AND MICROVISCOSITY IN RAT-LIVER UNDER OXIDATIVE STRESS

被引：14

作者：

BIAGI, PL

BORDONI, A

HRELIA, S

CELADON, M

TURCHETTO, E

机构：

[1] Department of Biochemistry G. Moruzzi, University of Bologna, Bologna

来源：

JOURNAL OF NUTRITIONAL BIOCHEMISTRY | 1993年 / 4卷 / 12期

关键词：

POLYENYLPHOSPHATIDYLCHOLINE; OXIDATIVE STRESS; DELTA-6-DESATURASE; FATTY ACIDS; MICROVISCOSITY; MALONDIALDEHYDE; LIVER MICROSOMES;

D O I：

10.1016/0955-2863(93)90108-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyenylphosphatidylcholine is a choline-glycerophospholipid containing up to 80% of total fatty acids as linoleic acid and may be an important factor in ensuring normal functioning of cell membranes. We tested the effect of a polyenylphosphatidylcholine-supplemented diet and compared it with both a trilinolein-supplemented and a laboratory chow diet on the fatty acid composition, microviscosity, and delta-6-desaturase activity of liver microsomal membranes of 12-month-old rats, in the absence or presence of oxidative stress induced by adriamycin. Polyenylphosphatidylcholine- and trilinolein-supplemented diets showed a similar increase in linoleic acid content and delta-6-desaturase activity in liver microsomes, indicating that low amounts of linoleic acid are able to partially restore the enzyme activity in old rats, independent of the source of linoleic acid. After adriamycin treatment, delta-6-desaturase activity increased in polyenylphosphatidylcholine and trilinolein groups, indicating a protective mechanism against the damage induced by polyunsaturated fatty acid peroxidation. The measurement of malondialdehyde production showed a protective effect on adriamycin-induced lipid peroxidation by polyenylphosphatidylcholine supplementation only. Microsomal membrane microviscosity did not change independent of diet and adriamycin treatment, suggesting that the response of microsomes to lipid peroxidation might be the maintenance of a given membrane order. Administration of polyenylphosphatidylcholine can prevent or minimize the liver damage induced by adriamycin treatment.

引用

页码：690 / 694

页数：5

共 28 条

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