STRUCTURE OF AN RNA DOUBLE HELIX INCLUDING URACIL-URACIL BASE-PAIRS IN AN INTERNAL LOOP

被引：131

作者：

BAEYENS, KJ ^{[1
]}

DEBONDT, HL ^{[1
]}

HOLBROOK, SR ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,LAWRENCE BERKELEY LAB,DIV STRUCT BIOL,BERKELEY,CA 94720

来源：

NATURE STRUCTURAL BIOLOGY | 1995年 / 2卷 / 01期

关键词：

D O I：

10.1038/nsb0195-56

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of the RNA dodecamer 5'-GGACUUUGGUCC-3' has been determined from X-ray diffraction data to 2.6 Angstrom resolution. This oligomer forms an asymmetric double helix in the crystal. Four consecutive non-Watson-Crick base-pairs are formed in the middle of the duplex including the first intrahelical U-U (or T-T) pairs observed in an oligonucleotide crystal structure. Two different conformations of U-U pairs are observed in the context of the surrounding sequence. One of these pairs is highly twisted, allowing a bound water to bridge across strands in the major groove. The crystal packing illustrates a new form of RNA helix-helix interaction.

引用

页码：56 / 62

页数：7

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