THE DETECTION OF LARGE MOLECULES IN MATRIX-ASSISTED UV-LASER DESORPTION

被引：59

作者：

SPENGLER, B

KIRSCH, D

KAUFMANN, R

KARAS, M

HILLENKAMP, F

GIESSMANN, U

机构：

[1] Institut für Lasermedizin, Düsseldorf, D-4000

[2] Institut für Medizinische Physik, Münster, D-4400, Hüfferstr

[3] Finnigan Mat, Bremen, D-2800

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1990年 / 4卷 / 09期

关键词：

D O I：

10.1002/rcm.1290040902

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mechanisms related to high‐mass ion detection were studied using matrix‐assisted UV laser desorption for ion generation and a tandem (MS/MS) time‐of‐flight mass spectrometer for the analysis of secondary species formed at the conversion dynodes. Secondary‐electron emission was found to be less pronounced with increasing molecular weight. Instead small secondary ions generated by collision of primary ions with the conversion dynodes appeared to be the dominant process for the detection of ions of masses up to several hundred thousand Da. The use of microchannel plate detectors has been shown to become less effective for high molecular weight applications. Mass spectra of secondary ions emitted from a conversion dynode bombarded with keV primary ions have been recorded. Regardless of the composition of the primary molecules, the secondary mass spectra were similar. Sputtering of surface adsorbates is assumed to be the major mechanism of secondary ion formation, rather than surface induced dissociation or fragmentation of the primary ions. Copyright © 1990 John Wiley & Sons Limited

引用

页码：301 / 305

页数：5

共 24 条

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